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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two additional splice variants ended up reported but thorough nucleotide sequence facts just isn't still obtainable.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide normally contains the epitope acknowledged with the antibody.
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This manual outlines even further doable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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